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引用本文:杨海君,谭菊. 入侵地加拿大一枝黄花根际和非根际土壤微生物群落结构及多样性[J]. 生物安全学报, .
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入侵地加拿大一枝黄花根际和非根际土壤微生物群落结构及多样性
杨海君, 谭菊
湖南农业大学植物保护学院
摘要:
为探明严重入侵地加拿大一枝黄花根际和非根际土壤微生物群落结构。采集长沙县黄华镇郭公渡严重地加拿大一枝黄花根际和非根际土壤,应用Illumina高通量测序技术研究了加拿大一枝黄花根际和非根际土壤微生物群落结构及多样性。结果表明:加拿大一枝黄花根际土壤有机质含量显著高于非根际土壤,二者间含水量差异不显著。在微生物群落多样性和丰度上,根际土壤细菌的Shannon指数和Simpson指数均大于非根际土壤,Chao1指数和ACE指数均小于非根际土壤,而根际土壤真菌的Shannon指数和Simpson指数均小于非根际土壤,Chao1指数和ACE指数均大于非根际土壤。在门水平上,酸杆菌门(Acidobacteriota)、变形菌门(Proteobacteria)和unidentified Bacteria的相对丰度均较高,三者在根际和非根际土壤样品中占比分别为69.9%和58.6%。子囊菌门(Ascomycota)在根际土壤和非根际土壤样品中真菌占比分别为58.6%和57.3%。在属水平上,根际土壤细菌中太阳念珠菌属(Candidatus Solibacter)、Ellin6067、伯克氏菌属(Burkholderia-Caballeronia-Paraburkholderia)和慢生根瘤菌属(Bradyrhizobium)的相对丰度明显高于非根际土壤,而不动杆菌属(Acinetobacter)的相对丰度显著低于非根际土壤。根际土壤真菌中的小画线壳属(Monographella)、鬼笔属(Phallus)、被孢霉属(Mortierella)、镰刀菌属(Fusarium)、斜盖伞属(Clitopilus)的相对丰度均明显高于非根际土壤,而Sistotrema、unidentified_Ascomycota_sp、unidentified_Paraglomerales_sp、茶渍属(Lecanora)的相对丰度明显低于非根际土壤。研究结果可为加拿大一枝黄花入侵机制研究提供理论支撑。
关键词:  加拿大一枝黄花  根际土壤微生物  非根际土壤微生物  高通量测序  土壤微生物群落结构
DOI:
基金项目:湖南省自然科学基金
相关附件:   万方20210516(16.79%)  附件1  附件2  附件3  附件1  附件2  附件1  附件2  附件3
Rhizosphere and non rhizosphere soil microbial structure and diversity of Solidago canadensis L. in the invaded land
Abstract:
The purpose of this study was to investigate the microbial community structure in the rhizosphere and non-rhizosphere soil of Solidago canadensis.We collected the rhizosphere and non-rhizosphere soil of Solidago canadensis from Guogongdu, Huanghua Town, Changsha County, and studied the microbial community structure and diversity in the rhizosphere and non-rhizosphere soil of Solidago canadensis using Illumina high-throughput sequencing technology.The results showed that the organic matter content in the rhizosphere soil of Solidago canadensis was significantly higher than that in the non-rhizosphere soil, but there was no significant difference in water content between them. In terms of diversity and abundance of microbial community, Shannon index and Simpson index of rhizosphere soil bacteria were higher than that of non-rhizosphere soil, while Chao1 index and ACE index were lower than that of non-rhizosphere soil bacteria by contrast.The Shannon index and Simpson index of fungi in rhizosphere soil were lower than those in non-rhizosphere soil, and the Chao1 index and ACE index were higher than those in non-rhizosphere soil. At the phylum level, the relative abundance of Acidobacteriota, Proteobacteria and Machimbacteria were all relatively high, accounting for 69.9% and 58.6% of the rhizosphere and non-rhizosphere soil samples, respectively. Ascomycota accounted for 58.6% and 57.3% of fungi in rhizosphere soil and non-rhizosphere soil samples, respectively. At the genus level, Candidatus in rhizosphere soil bacteria the relative abundance of Solibacter, Ellin6067, Burkholderia-Caballeronia-Paraburkholderia and Bradyrhizobium were significantly higher than that of non-rhizosphere soils. However, the relative abundance of Acinetobacter was obviously lower than that of non-rhizosphere soils. Monographella, Phallus, Mortierella, Fusarium and Clitopilus in the rhizosphere soil were significantly higher than those in the non-rhizosphere soil. The relative abundance of Sistotrema, unidentified_Ascomycota_sp, unidentified_Paraglomerales_sp and Lecanora were significantly lower than that of non-rhizosphere soils. These results can provide theoretical support for the study on the invasion mechanism of Solidago canadensis.
Key words:  Solidago canadensis L.  rhizosphere soil microorganism  non rhizosphere soil microorganism  high throughput sequencing  microbial community structure of soil