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引用本文:宁静,张宾,田浩楷,柳小龙,杨炳琰,赵莉蔺. 携带与非携带松材线虫的松墨天牛miRNA表达谱比较分析[J]. 生物安全学报, 2018, 27(1): 20-30.
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携带与非携带松材线虫的松墨天牛miRNA表达谱比较分析
宁静1,2, 张宾2, 田浩楷2,3, 柳小龙2,4, 杨炳琰2,3, 赵莉蔺2
1.河北大学生命科学学院, 河北 保定 071002;2.中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101;3.中国科学院大学, 北京 100049;4.山西农业大学林学院, 山西 太谷 030801
摘要:
[目的]松墨天牛是松树的重要蛀干害虫,也是林业重大外来入侵种松材线虫的媒介昆虫。虽然松墨天牛和松材线虫互作的化学生态和分子进化机制受到人们的广泛关注,但miRNA等表观遗传因子在天牛-线虫互作中的作用未见报道。[方法]使用illuminaHiSeq 2000平台进行miRNA高通量测序,得到4个携带线虫的天牛miRNA库和4个未携带线虫的天牛miRNA库。此外,对鉴定出的miRNA进行了差异表达分析,并对这些miRNA的靶基因进行了GO注释和KEGG通路富集分析。[结果]在携带线虫的天牛表皮、脂肪体、中肠和气管样本中分别鉴定出780、802、617和762个miRNA;在未携带松材线虫的天牛的不同组织样本中分别鉴定出784、723、713和837个miRNA。在携带松材线虫的松墨天牛中,某些已知miRNA表达量会显著升高,如miR-14、miR279和miR-312等。差异表达miRNA的功能大多指向代谢、免疫等方面。[结论]miRNA在松墨天牛和松材线虫互作中起着重要的调控作用。
关键词:  松墨天牛  松材线虫  共生互作  miRNA  高通量测序
DOI:10.3969/j.issn.2095-1787.2018.01.003
基金项目:国家重点研发计划(2016YFC1200600);国家自然科学基金(31572272);中国科学院创新工程项目(KSCX2-EW-J-2);中国科学院前沿科学重点研究项目(QYZDB-SSW-SMC014)
附件
Comparative analysis of microRNA profile in Monochamus alternatus in the presence or absence of the pinewood nematode, Bursaphelenchus xylophilus
NING Jing1,2, ZHANG Bin2, TIAN Haokai2,3, LIU Xiaolong2,4, YANG Bingyan2,3, ZHAO Lilin2
1.College of Life Sciences, Hebei University, Baoding, Hebei 071002, China;2.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Abstract:
[Aim] Monochamus alternatus, has established a symbiotic relationship with, and is the main vector of the pinewood nematode, Bursaphelenchus xylophilus. However, little is known about the symbiotic relationship at the molecular level. microRNAs (miRNAs) are considered to be very important in regulating the growth, development and behavior in animals and plants in post-transcriptional gene regulation.[Method] Eight small RNA libraries were constructed and sequenced by the illumina high-throughput sequencing technology. In addition, differential expression of the identified miRNAs was analyzed, and the function of target genes for these miRNA were predicted by GO annotation and KEGG pathway enrichment analysis.[Result] From these data we identified known miRNAs from the library of beetle's epidermis, fat body, midgut and trachea with nematodes (780+802+617+762), and without nematodes (784+723+713+837). The prediction of target genes function for these miRNAs suggested that miRNAs might participate in metabolism regulation and the immune response of the beetle host.[Conclusion] miRNA plays an important regulatory role in the interaction between M. alternatus and its nematode sybmiont.
Key words:  Monochamus alternatus  Bursaphelenchus xylophilus  symbiosis  miRNA  high-throughput sequencing