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引用本文:吴咚咚,赵建伟,陈颖,郑宇,何玉仙. 辣椒碱对烟粉虱体内羧酸酯酶、谷胱甘肽S-转移酶和乙酰胆碱酯酶活性的影响[J]. 生物安全学报, 2012, 21(2): 109-113.
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辣椒碱对烟粉虱体内羧酸酯酶、谷胱甘肽S-转移酶和乙酰胆碱酯酶活性的影响
吴咚咚1, 赵建伟2, 陈颖3, 郑宇2, 何玉仙2
1.福州市农业科学研究所,福建 福州 350018;2.福建省农业科学院植物保护研究所,福建 福州 350013;3.泉州市农业局,福建 泉州 362000
摘要:
【背景】烟粉虱作为世界性的多食性害虫,对我国农业生产造成了严重危害。已有研究表明,许多植物的次级代谢产物能够对烟粉虱解毒酶和靶标酶活性产生影响。【方法】采用常规生化方法研究了辣椒碱对烟粉虱体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和乙酰胆碱酯酶(AChE)活性的影响。【结果】与对照相比,500 mg·L-1辣椒碱处理烟粉虱成虫后6 h,CarE活性被激活,其他时段被抑制;1000 mg·L-1辣椒碱处理后6、12、48 h,CarE活性受到抑制,其他时段被激活;2000 mg·L-1辣椒碱处理后6 h,CarE活性受到抑制,其他时段被激活;4000 mg·L-1辣椒碱处理后3、12 h,CarE活性受到抑制,其他时段被激活;8000 mg·L-1辣椒碱处理后1 h,CarE活性被激活,其他时段受到抑制。辣椒碱对烟粉虱体内GSTs活性的诱导存在剂量和时间效应,8000 mg·L-1辣椒碱处理烟粉虱成虫后24和48 h, GSTs活性受到明显抑制,其余各处理在任何时段GSTs活性均被激活而高于对照。辣椒碱各浓度处理对烟粉虱体内AChE活性的诱导存在时间效应,各处理均在处理后48 h对AChE活性产生了抑制作用。【结论与意义】辣椒碱对烟粉虱体内的解毒酶CarE和GSTs、靶标酶AChE活性具有明显影响,且存在剂量或时间效应,这可为辣椒碱在生物农药领域的应用提供理论依据。
关键词:  辣椒碱  烟粉虱  入侵昆虫  羧酸酯酶  谷胱甘肽S 转移酶  乙酰胆碱酯酶  生物防治
DOI:10.3969/j.issn.2095-1787.2012.02.006
基金项目:农业公益性行业科研专项(200803005); 福建省科技重点项目(2008N0114); 福建省财政专项——福建省农业科学院科技创新团队建设基金项目(STIF-Y07)
附件
Effects of capsaicin on the activities of carboxylesterase, glutathione-S-transferase and acetylcholinesterase in Bemisia tabaci
Dong-dong WU1, Jian-wei ZHAO2, Ying CHEN3, Yu ZHENG2, Yu-xian HE2
1.Institute of Agricultural Sciences of Fuzhou, Fuzhou, Fujian 350018, China;2.Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;3.Agriculture Bureau of Quanzhou, Quanzhou, Fujian 362000, China
Abstract:
【Background】The sweet potato whitefly, Bemisia tabaciGennadius, is one of the most important pests worldwide, and has caused serious damage to agriculture production in China. Plant secondary metabolites may significantly affect the activity of several enzymes involved in detoxification, including carboxylesterase (CarE), glutathione-S-transferase (GSTs) and acetylcholinesterase (AChE) in B.tabaci. 【Method】The effects of capsaicin on activities of CarE, GSTs and AChE in B.tabaci were tested by conventional biochemical methods. 【Result】After exposed to 500 mg·L-1 capsaicin; CarE was activated at 6 h, but was inhibited at other times when the capsaicin concentration was increaed to 1000 mg·L-1, the activity was inhibited at 6 h, 12 h and 48 h, but was activated at other times; at 2000 mg·L-1, the activity was inhibited at 6 h, but was activated at other times. Treatment with 4000 mg·L-1capsaicin caused inhibition at 3 h and 12 h, but activation at other times; at 8000 mg·L-1, the enzyme was activated at 1 h, but inhibited at other times. At a concentration of 8000 mg·L-1, the activity of GSTs was inhibited significantly at 24 h and 48 h, but was activated at other times and other concentrations. In the case of AchE, the activity inhibition happened at 48 h after every concentration of capsaicin application. 【Conclusion and significance】The capsaicin may significantly affect the enzyme activities of CarE, GSTs and AChE in B.tabaciwith the dose or time dependent effects. This study could provide a basis for capsaicin application against B.tabaci.
Key words:  capsaicin  Bemisia tabaci  invasive insect  carboxylesterase  glutathione-S-transferase  acetylcholinesterase  biological control